Human Influenza Virus Real-time RT-PCR: Detection and Discrimination of Influenza A (H3N2) Variant from Seasonal Influenza A (H3N2) Viruses, Including H3v and Seasonal H3 Assays

This invention relates to methods of rapidly detecting influenza, including differentiating between type and subtype. CDC researchers have developed a rapid, accurate, real-time RT-PCR assay that has several advantages over culture and serological tests, which require 5 to 14 days for completion; this assay can also be easily implemented in kit form. To date, hundreds of human cases of infection with the H3N2 variant virus have been confirmed. The increased numbers of human infection of H3N2 variant virus has led to a need for a highly sensitive and specific assay for the diagnosis and confirmation of the H3N2 variant virus.

Potential Commercial Applications: Competitive Advantages:
  • Influenza diagnostic using clinical specimens
  • High-throughput sample screening
  • Government, regional influenza surveillance programs
  • Especially useful for H3N2 screening
  • Sensitive detection
  • Specific discrimination of influenza subtypes
  • Easily formatted as kit or array
  • Faster than culturing and serological identification methods
  • Less laborious and more objective than immunoassays

Development Stage:
In vitro data available

Related Invention(s):


Stephen Lindstrom (CDC)  ➽ more inventions...

Bo Shu (CDC)  ➽ more inventions...

Kai-Hui Wu (CDC)  ➽ more inventions...

LaShondra Berman (CDC)  ➽ more inventions...

Intellectual Property:
US Pat: 10,006,097 issued 2018-06-26
US Application No. 61/894,291 filed on 2013-10-22
PCT Application No. PCT/US2014/061802 filed on 2014-10-22
US Application No. 15/989,924 filed on 2018-05-25

Lindstrom S, et al. PMID 22516540
Cox CM, et al. PMID 21749798
Jhung MA, et al. PMID 24065322

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Phone: 301-594-9719

OTT Reference No: E-562-2013-0
Updated: Jul 25, 2018