Transgenic Mice with Constitutively Active M3 Muscarinic Receptor in Islet Beta Cells


Q490L point mutation was introduced into the rat M3 muscarinic receptor cDNA to confer persistent, constitutive (ligand-independent) activity. Expression of the M3 receptor mutant was placed under the control of a 650 bp fragment of the rat insulin promoter II (RIP II) to limit expression to the islet beta cell.

Potential Commercial Applications: Competitive Advantages:
Diabetes research, especially type II Diabetes. Beneficial metabolic effects of this mouse model include high basal insulin secretion, improved glucose tolerance, increased serum insulin, and resistance to high-fat diet-induced glucose intolerance and hyperglycemia.


Development Stage:
In vivo data available (animal)

Inventors:

Jurgen Wess (NIDDK)  ➽ more inventions...


Intellectual Property:
Research Tool — Patent protection is not being pursued for this technology.

Publications:
Gautam D, et al. PMID 20843999

Licensing Contact:
Mythreyi Shastri, Ph.D.
Email: shastrim@mail.nih.gov
Phone: 301-435-0613

OTT Reference No: E-453-2013-0
Updated: Mar 24, 2017