Transgenic Hspa2-Cre Mice for Studying Spermatogenesis and Male Infertility

HSPA2 is a member of the HSP70 family of heat-shock proteins that serve as molecular chaperones. Hspa2-cre expression mimics the spermatogenic cell-specific expression of endogenous HSPA2 within the testis, being first observed in leptotene/zygotene spermatocytes. Expression of the transgene is also detected at restricted sites in the brain, as occurs for endogenous HSPA2.

Researchers at NIEHS developed the first transgenic mouse line that expresses Cre-recombinase under the control of the promoter of the heat shock protein A2 (Hspa2) gene. Expression of the Hspa2-Cre transgene during meiosis in male germ cells makes these mice a useful tool for defining the roles of genes expressed at different times during spermatogenesis or expressed in spermatogenic cells.

Potential Commercial Applications: Competitive Advantages:
  • New mouse model to study spermatogenesis and male infertility
  • New mouse model to study meiosis or the roles of heat-shock proteins in general
  • Researchers generated an Hspa2-cre line that expresses cre in spermatocytes to overcome the limitations of other transgenic lines.

Development Stage:
  • In vitro data available
  • In vivo data available (animal)

Related Invention(s):


Edward Eddy (NIEHS)  ➽ more inventions...

Intellectual Property:
Research Tool — Patent protection is not being pursued for this technology.

Inselman AL, et al. PMID 20027617

Collaboration Opportunity:

The NIEHS is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize this mouse strain. For collaboration opportunities, please contact Sharon Soucek, Ph.D. at

Licensing Contact:
Vidita Choudhry, Ph.D.
Phone: 301-594-4095

OTT Reference No: E-290-2011-0
Updated: Sep 4, 2020