Simultaneous Detection of Non-pneumophila Legionella Strains Using Real-time PCR

Legionnaires' disease is caused by a type of bacteria called Legionella. CDC scientists have developed a real-time multiplex PCR assay for diagnosis and identification of Legionella strains. The assay consists of five sets of primers (targeting L. bozemanii, L. dumoffii, L. feeleii, L. longbeachae, or L. micdadei) and corresponding probes. Each probe is labeled with a different fluorophore which allows the detection of a particular strain in a single tube reaction. Using this assay format, the presence of any one of the five pathogenic non-pneumophila strains of Legionella can be detected rapidly from clinical or environmental samples. Rapid and sensitive identification enables initiation of appropriate antibiotic therapy and identification of the source of bacteria so that proper public health responses may occur.

Potential Commercial Applications: Competitive Advantages:
  • Rapid and real-time assay to detect the presence of clinically relevant non-pneumophila Legionella strains.
  • Currently available tests are time consuming and labor intensive.
  • This assay enables rapid identification and differentiation on clinically relevant non-pneumophila Legionella strains.
  • This assay can be used as a standalone confirmatory assay for the detection of common non-pneumophila Legionella species or as one of the valuable assays in conjunction with other standard assays.

Development Stage:
  • Pre-clinical
  • In vitro data available


Jonas Winchell (CDC)  ➽ more inventions...

Alvaro Benitez (CDC)  ➽ more inventions...

Intellectual Property:
U.S. Pat: 10,202,654 issued 2019-02-12
U.S. Pat: 9,394,574 issued 2016-07-19
PCT Application No. PCT/US2013/030217
US Application No. 61/658,627
PCT Application No. PCT/US2014/038441
US Application No. 14/407,347

Benitez AJ, Winchell JM. PMID 23135949

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Phone: 301-594-9719

OTT Reference No: E-277-2013-0
Updated: Aug 2, 2013