Multiplex Real-time PCR Assay for Detection of Numerous Bacterial Pathogens

In order to address a global need for rapid, cost-effective, sensitive, and specific assays for many pathogens, CDC scientists have developed a broad-use, multiplexed RT-PCR assay. This comprehensive assay covers numerous pathogens that are common causes of infection in neonates and also important to food-safety. Specifically, this assay (and respective probes, primers, and kits) is capable of detecting one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis in a biological sample.

Potential Commercial Applications:		Competitive Advantages:
Clinical diagnostic for several pathogens Drug-resistance surveillance Public health monitoring En masse food-safety screening		Cost-effective Simple to implement Rapid, accurate and objectively conclusive Easily implemented into kit format Ideal for high-throughput scenarios

Development Stage:

• Pre-clinical
• In vitro data available

Inventors:

Jonas Winchell (CDC)  ➽ more inventions...

Bernard Wolff (CDC)  ➽ more inventions...

Maureen Diaz (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 10,072,305 issued 2018-09-11
PCT Application No. PCT/US2013/028034
US Application No. 14/398,390
US Application No. 16/056,147

Publications:
Diaz MH, et al. PMID 23805203

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-276-2013-0
Updated: Sep 18, 2013