Total Emission Detection System for Multi-Photon Microscopy

Available for licensing and commercial development is a novel two-photon microscope system, which would allow improved fluorescent light collection, the use of less excitation power and deeper penetration of tissue and isolated cells. Multi-photon fluorescence microscopy (MPFM) is an imaging technique that can investigate biological processes to sub-cellular resolution at depths of hundreds of microns below the surface of biological tissues. MPFM provides higher resolution imaging of tissues than confocal imaging, but is currently limited by the use of inefficient light collection systems, which lead to detection of only a fraction of the light that is emitted from the sample. The new system consists of an array of mirrors, lenses, and reflecting surfaces designed to collectively maximize the probability of collecting all emitted fluorescent light to a detector, thereby providing enhanced brightness of light detected from the sample and an increase in signal-to-noise ratio (SNR). This increase is SNR can be used to improve time resolution, reduce laser power requirements and reduce photodynamic damage.

Potential Commercial Applications: Competitive Advantages:
  • Three-dimensional imaging of biological tissues and cells
  • Three-dimensional imaging of semiconductor integrated circuits

Development Stage:
Late-stage technology


Christian Combs (NHLBI)  ➽ more inventions...

Robert Balaban (NHLBI)  ➽ more inventions...

Jay Knutson (NHLBI)  ➽ more inventions...

Intellectual Property:
U.S. Pat: 7,667,210 issued 2010-02-23
PCT Application No. PCT/US2007/17478
US Application No. 60/835,462

Collaboration Opportunity:

The NHLBI Light Microscopy Core Facility is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize a total emission detection system for multi-photon imaging. Please contact Alan Deutch, Ph.D., Director of the NHLBI Office of Technology Transfer and Development, at 301-402-5579 or via Email at for more information.

Licensing Contact:
Michael Shmilovich, J.D.
Phone: 301-435-5019

OTT Reference No: E-257-2005-0
Updated: May 1, 2018