Polyclonal Antibodies to NKCC2, a Kidney-Specific Member of the Cation Chloride Co-transporter Family, SLC12A1


Antibodies to NKCC2, useful for immunoblotting and immunocytochemistry, are available to resell for research purposes. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle, where it facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell. Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is blocked by loop diuretics such as furosemide. The inventor has developed rabbit polyclonal antibodies directed against a peptide sequence in the N-terminal tail of NKCC2.

Potential Commercial Applications: Competitive Advantages:
Western blotting and immunocytochemistry 


Inventors:

Mark Knepper (NHLBI)  ➽ more inventions...


Intellectual Property:
Research Tool -- patent protection is not being pursued for this technology

Publications:
GH Kim, CA Ecelbarger, C Mitchell, RK Packer, JB Wade, MA Knepper. Vasopressin increases Na-K-2CI cotransporter expression in thick ascending limb of Henle's loop. Am J Physiol. 1999 Jan;276(1 Pt 2):F96-F103. PubMed abs
HL Brooks, AJ Allred, KT Beutler, TM Cofiman, MA Knepper. Targeted proteomic profiling of renal Na+ transporter and channel abundances in angiotensin II type 1a receptor knockout mice. Hypertension. 2002 Feb;39(2 Pt 2):470-473. PubMed abs

Licensing Contact:
Michael Shmilovich, J.D.
Email: shmilovm@mail.nih.gov
Phone: 301-435-5019

OTT Reference No: E-255-2008-0
Updated: May 5, 2009