Genome Wide DNase I Hypersensitive Sites Detection in Formalin-Fixed Paraffin-Embedded Single Cells
A method of detecting DNase I hypersensitive sites ((DHS) in a single cell or very small number of cells, including cells recovered from formalin-fixed paraffin-embedded (FFPE) tissue slides of patient samples. DHS has revealed a large number of potential regulatory elements for transcriptional regulation in various cell types. The application of DNase-Seq techniques to patient samples can elucidate pathophysiological mechanisms of gene function in a variety of diseases as well as provide potentially important diagnostic and prognostic information. Unfortunately, the current DNase-Seq techniques require large number of cells and are applicable only to larger biopsies and surgical specimens. This technique, called Pico-Seq, allows detection when only very small population of cells are available, such as rare primary tumor cells and circulating-tumor-cells, isolated by a variety of methods. Pico-Seq uses conditions capable of restoring the DNase I sensitivity, similar to native/fresh cells, in tissue/cells from slides processed by extremely harsh conditions, such as in FFPE tissues.
Potential Commercial Applications: | Competitive Advantages: |
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Development Stage:
- Early-stage
- In vitro data available
Inventors:
Keji Zhao (NHLBI) ➽ more inventions...
Qingsong Tang (NHLBI) ➽ more inventions...
Intellectual Property:
US Application No. 62/118,574
Licensing Contact:
Admin. Licensing Specialist (ALS),
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OTT Reference No: E-254-2014-0
Updated: Aug 17, 2015