An In-Vitro Cell System Useful For Identification of RORgamma Antagonists


The retinoid-related orphan receptors alpha, beta and gamma (RORalpha, beta and gamma , also referred to as NR1F1, 2 and 3, respectively) comprise a distinct subfamily of nuclear receptors. Study of ROR-deficient mice has implicated RORs in the regulation of a number of biological processes and revealed potential roles for these proteins in several pathologies. NIH investigators have developed an in-vitro system using CHO cells stably expressing a TET-On expression vector regulating RORgamma and a RORE-Luciferase reporter. This system allows inducible expression of RORgamma upon addition of doxycycline. Upon its induction RORgamma binds to the RORE in the luciferase reporter plasmid and induces luciferase. This system can be used to identify RORgamma antagonists. This system has been tested successfully in 1536-well plate high throughput analysis.

Potential Commercial Applications: Competitive Advantages:
  • Identification of therapeutic compounds to treat asthma, inflammation, and various autoimmune diseases such as osteoarthritis, multiple sclerosis.
 
  • Novel and unique system to screen and identify chemical and drugs for their RORgamma antagonistic activity.


Development Stage:
  • Early-stage
  • Pre-clinical
  • In vitro data available


Related Invention(s):
E-222-2009-0


Inventors:

Anton Jetten (NIEHS)  ➽ more inventions...

Yukimasa Takeda (NIEHS)  ➽ more inventions...


Intellectual Property:
Research Tool — Patent protection is not being pursued for this technology.

Publications:
Jetten AM. PMID 19381306
Yang XO, et al. PMID 18164222
Kurebayashi S, et al. PMID 10963675

Collaboration Opportunity:

The NIEHS, Laboratory of Respiratory Biology, Cell Biology Group, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize retinoid-related orphan receptors (RORs) function in chronic diseases. For collaboration opportunities, please contact Sharon Soucek, Ph.D. at sharon.soucek@niehs.nih.gov.


Licensing Contact:
Vidita Choudhry, Ph.D.
Email: vidita.choudhry@nih.gov
Phone: 301-594-4095

OTT Reference No: E-253-2010-0
Updated: Sep 4, 2020