Fluorescent Primer(s) Creation for Nucleic Acid Detection and Amplification

CDC researchers have developed technology that consists of a simple and inexpensive technique for creating fluorescent labeled primers for nucleic acid amplification. Fluorescent chemical-labeled probes and primers are extensively used in clinical and research laboratories for rapid, real-time detection and identification of microbes and genetic sequences. During nucleic acid amplification, the "UniFluor" primer is incorporated into newly synthesized double stranded DNA. As a consequence, quenching of the dye's fluorescent signal occurs decreasing the fluorescence of the sample several fold. The decrease in fluorescence can be measured and observed using any commercially available nucleic acid amplification system that measures fluorescence (e.g., real-time PCR/thermocyclers). Because many real-time PCR applications require a multitude of fluorescently labeled primers or probes, the single-labeled primer technique also allows researchers and clinicians to perform their work at lower cost.

Potential Commercial Applications: Competitive Advantages:
  • Quantitative detection and/or amplification of specified nucleic acid sequences
  • Efficient fluorescence-labeling of oligonucleotides
  • Pyro-sequencing
  • Basic laboratory research
  • Simple to implement
  • Rapid, real-time detection
  • Used with standard laboratory equipment capable of monitoring fluorescence-intensity shifts
  • Cost-effective
  • Easily adapted for use in kits or arrays

Development Stage:
In vitro data available

Related Invention(s):


Jothikumar Narayanan (CDC)  ➽ more inventions...

Vincent Hill (CDC)  ➽ more inventions...

Intellectual Property:
US Pat: 7,709,626 issued 2010-05-04
PCT Application No. PCT/US2006/000175 filed on 2006-01-03
US Application No. 60/641,303 filed on 2005-01-03
US Application No. 12/267,869 filed on 2006-01-03
Several international patent applications pending or issued

Licensing Contact:
Jeremiah Mitzelfelt,
Email: jeremiah.mitzelfelt@nih.gov
Phone: 301-443-8518

OTT Reference No: E-252-2013-0
Updated: Mar 9, 2020