Super-Resolution Fluorescence Enhanced Imaging using Bleaching/Blinking Assisted Localization Microscopy (BALM)

The invention relates to systems and methods for localization microscopy for superresolution imaging of fluorescent molecules. The method utilizes intrinsic bleaching/blinking properties of fluorophores in which superresolution is achieved by capturing successive images and subtracting from each either the subsequent image. The location of a single fluorescent molecule can be identified when the molecules either photobleach, blink off, or blink between successive images using a higher magnification lens to achieve a smaller pixel size.

Potential Commercial Applications: Competitive Advantages:
  • Tissue imaging
  • Cell structure imaging
 Higher magnification at lower pixel size

Development Stage:
  • Prototype
  • In vitro data available


Bechara Kachar (NIDCD)  ➽ more inventions...

Intellectual Property:
US Application No. 61/784,266

Licensing Contact:
Michael Shmilovich, J.D.
Phone: 301-435-5019

OTT Reference No: E-247-2011-0
Updated: Jun 6, 2013