Mouse IL-12p40 Expressing Cell Line


The subject invention is a recombinant human 293T cell line that expresses mouse IL-12p40 protein to high levels. IL-12p40 is a subunit of both Interleukin-12 (IL-12) and IL-23; however, it can also be expressed as a monomer (IL-12p40) and as a homodimer (IL-12p80). IL-12p40 is produced mainly by antigen presenting cells such as macrophages, neutrophils, microglia, and dendritic cells in response to pathogens or inflammatory agents. It is an immunostimulatory messenger molecule that can disseminate in the body and signal the presence of a pathogen. The role of IL-12p40 is still being elucidated. This cell line produces and secretes mouse IL-12p40 proteins that have post-translational modifications similar to native IL-12p40 protein, overcoming an issue that is seen with IL-12p40 protein expressed in bacterial, insect, or hamster cells.

Potential Commercial Applications: Competitive Advantages:
Production of mouse IL-12p40 for research applications. IL-12p40 protein is expressed in human cell line, so post-translational modifications are similar to native protein.


Development Stage:
In vitro data can be provided upon request.

Inventors:

Nevil Singh (NIAID)  ➽ more inventions...


Intellectual Property:
Research Tool — Patent protection is not being pursued for this technology.

Licensing Contact:
Barry Buchbinder, Ph.D.
Email: BBuchbinder@niaid.nih.gov
Phone: 301-496-2644

OTT Reference No: E-247-2010-0
Updated: May 20, 2011