Resolution Enhancement for Light Sheet Microscopy Systems

The invention pertains to a technique for enhancing the resolution of images in light sheet microscopy by adding additional enhanced depth-of-focus optical arrangements and high numerical aperture objective lenses. The technique employs an arrangement of three objective lenses and a processor for combining captured images. The image composition utilizes the greater resolving power of the third high numerical aperture objective lens by imaging the light sheet and enhanced depth-of-focus arrangement resulting in improved overall resolution of the light sheet system. The depth of field arrangement could be a simple oscillation of the third objective, a "layer cake," or cubic phase mask component. Any loss in lateral resolution that results from the depth of field arrangement may be compensated for by deconvolution. In some embodiments, other optics, such as an axicon or annular aperture, can provide extended depth of field.

Potential Commercial Applications: Competitive Advantages:
  • High speed imaging
  • Fast single cell and cellular dynamics imaging
  • Superresolution and single molecule imaging
  • 3D single particle tracking
  • 3D superresolution imaging in thick samples
  • Resolution enhancement in light microscopy

Development Stage:
In vitro data available

Related Invention(s):


Hari Shroff (NIBIB)  ➽ more inventions...

Yicong Wu (NIBIB)  ➽ more inventions...

Sara Abrahamsson

Intellectual Property:
U.S. Pat: 10,401,604 issued 2019-09-03
US Application No. 62/054,484
US Application No. 15/511,580

Collaboration Opportunity:

The National Institute of Biomedical Imaging and Bioengineering is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize Resolution Enhancement Technique for Light Sheet Microscopy Systems. For collaboration opportunities, please contact Cecilia Pazman at 301-594-4273 or

Licensing Contact:
Michael Davis, Ph.D., J.D.
Phone: 301-451-9032

OTT Reference No: E-232-2014-0
Updated: Jun 29, 2015