Species-specific Nucleic Acid Detection Assay for Fungi

This invention pertains to nucleic acid-based assays for the detection of Aspergillus and other filamentous fungi. Assays cover the species-specific detection and diagnosis of infection by Aspergillus, Fusarium, Mucor, Penecillium, Rhizomucor, Absidia, Cunninghamella, Pseudallescheria or Sporthrix in a subject. This can reduce identification time from several days by conventional culture methods to a matter of hours. Furthermore, genus-specific probes are also provided for Aspergillus, Fusarium and Mucor, in addition to an "all-fungus" nucleic acid probe. This technology is readily adaptable as kits used for species-specific identification of opportunistic pathogen infections or possible work/home contamination.

Potential Commercial Applications: Competitive Advantages:
  • Directing antifungal drug therapy for improved patient outcomes
  • Detection, discrimination of fungal species from biological samples
  • Addressing secondary infections of immunosuppressed individuals or asthmatics
  • Rapid, sensitive, simple and specific
  • Cost-efficiency compared to culture or sero-diagnostic methods
  • Easily adaptable to kit form
  • High-throughput screening

Development Stage:
In vitro data available

Related Invention(s):


Christine Morrison (CDC)  ➽ more inventions...

Errol Reiss (CDC)  ➽ more inventions...

Lilianna Aidorevich (CDC)  ➽ more inventions...

Jong Soo Choi (CDC)  ➽ more inventions...

Intellectual Property:
PCT Pat: 6,372,430 issued 2002-04-16
US Pat: 7,052,836 issued 2006-05-30
US Application No. 09/423,233 filed on 2000-06-27
US Application No. 10/046,955 filed on 2002-01-14

Licensing Contact:
Jeremiah Mitzelfelt,
Email: jeremiah.mitzelfelt@nih.gov
Phone: 301-443-8518

OTT Reference No: E-232-2013-0
Updated: Dec 23, 2013