Resolution Enhancement for Line-Scanning Excitation Microscopy

The invention describes a method for improving the spatial resolution of optical microscopes that use line-scanning excitation, such as line-scanning confocal microscopes, line-scanning STED microscopes, or line-scanning light-sheet microscopes. Common elements of the invention include: a) an apparatus for exciting and scanning a line-like excitation focus through the sample; b) an optical arrangement on the detection side of the microscope for manipulating the spacing and/or width of the resulting fluorescence emissions; c) integration and optional post-processing of the manipulated fluorescence emissions after capture by an area detector such as a camera. The resolution increase may be performed with no or marginal decrease in temporal resolution relative to the conventional line-scanning microscopes upon which the technique is based.

Potential Commercial Applications: Competitive Advantages:
  • Fluorescence microscopy
  • Improved resolution
  • Enhanced acquisition speed relative to other forms of super-resolution microscopy

Development Stage:


Hari Shroff (NIBIB)  ➽ more inventions...

Andrew York (NIBIB)  ➽ more inventions...

John Giannini (NIBIB)  ➽ more inventions...

Abhishek Kumar (NIBIB)  ➽ more inventions...

Intellectual Property:
PCT Pat: 10,247,930 issued 2019-04-02
US Application No. 62/054,481 filed on 2014-09-24
US Application No. 15/512,870 filed on 2017-03-21
US Application No. 16/371,641 filed on 2019-04-01

Collaboration Opportunity:

The National Institute of Biomedical Imaging and Bioengineering is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize fluorescent microscopy. For collaboration opportunities, please contact Cecilia Pazman at 301-594-4273 or

Licensing Contact:
Michael Davis, Ph.D., J.D.
Phone: 301-451-9032

OTT Reference No: E-225-2014-0
Updated: Oct 27, 2014