Real-Time PCR for Detecting Legionella Species and Discriminating Legionella pneumophila

Legionella pneumophila is the causative species in most cases of Legionnaires' disease (LD). CDC scientists have developed a real-time PCR assay capable of detecting all Legionella species and discriminating L. pneumophila from other Legionella species. LD is typically difficult to diagnose from a clinical standpoint as it confers no unique clinical features or symptoms. This assay provides a rapid and accurate alternative to laborious PCR assays, prone to aberrant results. It provides a sensitive alternative for diagnosis of Legionnaires' disease and detection of L. pneumophila.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostic for Legionnaires’ disease
  • Detection of all Legionella species and specific discrimination of L. pneumophila
  • Faster than immunoassays
  • Less laborious than current LD diagnostics
  • Rapid, sensitive, and specific
  • Curtails misdiagnoses associated with serological evaluations
  • Easily adaptable to kit form

Development Stage:
  • Early-stage
  • In vitro data available


Robert Benson (CDC)  ➽ more inventions...

Brian Holloway (CDC)  ➽ more inventions...

Karen McCaustland (CDC)  ➽ more inventions...

Patrick Yang (CDC)  ➽ more inventions...

Intellectual Property:
US Application No. 61/138,727
PCT Application No. PCT/US2009/068461
US Application No. 13/140,922

Yang G, et al. PMID 19438641

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Phone: 301-594-9719

OTT Reference No: E-194-2013-0
Updated: Sep 19, 2013