Real-time PCR Assays for Selective Detection and Differentiation of B. pertussis, B. parapertussis and B. homesii


CDC researchers developed a real-time PCR assay targeting insertion sequence (IS481) and pertussis toxin subunit 1 (ptxS1) of Bordetella pertussis. This real-time nucleic acid assay offers rapid, sensitive, and quantitative results. The employed primers have been validated through extensive diagnostic testing of 41 Bordetella and 64 non-Bordetella clinical isolates. This technology can be used to diagnose and distinguish B. pertussis, B. parapertussis and B. homesii, the three most common Bordetella human upper respiratory pathogens. A standalone assay or multi-faceted kit may be used.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostics for Bordetella pathogens
  • Investigation of acute upper respiratory illness and outbreaks
 
  • Validated for the three major pathogens responsible for Bordetella-related upper respiratory infections
  • Rapid, sensitive and quantitative
  • Easily adapted to kit form
  • Useful as an added, internal control for present Bordetella pertussis diagnostics


Development Stage:
  • Early-stage
  • In vitro data available


Inventors:

Kathleen Tatti (CDC)  ➽ more inventions...

Kansas Sparks (CDC)  ➽ more inventions...

Maria-Lucia Tondella (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 9,096,908 issued 2015-08-04
PCT Application No. PCT/US2010/032408
US Application No. 13/266,099

Publications:
Tatti KM, et al. PMID 18440175

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-193-2013-0
Updated: Sep 17, 2013