Polyclonal Antibodies for the Specialized Signaling G protein, Gbeta5


Researchers at NIDDK have developed polyclonal antibodies against the G protein, Gbeta5. Gbeta5 is a unique and highly specialized G protein that exhibits much less homology than other Gbeta isoforms (~50%) and is preferentially expressed in brain and neuroendocrine tissue. It is expressed prominently in the neuronal cell membrane, as well as in the cytosol and nucleus. Although this distribution pattern suggests that Gbeta5 may shuttle information between classical G protein-signaling elements at the plasma membrane and the cell interior, its function in the brain is largely unknown.

The antibodies were separately generated in rabbits to KLH-conjugates of peptides from the N-terminus of Gbeta5 (antibody ATDG) and the C-terminus of Gbeta5 (antibody SGS). The antibodies can be used for immunoblotting (ATDG, SGS), and immunoprecipitation (ATDG). They can be used to facilitate our understanding of the unique biology and function of Gbeta5 in brain and neurons.

Potential Commercial Applications: Competitive Advantages:
These antibodies can be used for research purposes (immunoblotting, immunoprecipitation) by those studying the biology and function of Gbeta5. Very specific antibodies to study Gbeta5 and G protein signaling.


Development Stage:
In vitro data available

Inventors:

William Simonds (NIDDK)  ➽ more inventions...

Jianhua Zhang (NIDDK)  ➽ more inventions...


Intellectual Property:
Research Tool — Patent protection is not being pursued for this technology.

Publications:
Zhang JH and Simonds WF. PMID 10648734
Zhang JH, et al. PMID 11152459
Zhang S, et al. PMID 8969224
Zachariou V, et al. PMID 16415864

Licensing Contact:
Betty Tong, Ph.D.
Email: tongb@niddk.nih.gov
Phone: 301-451-7836

OTT Reference No: E-192-2006-0
Updated: Jul 27, 2012