Real-time TaqMan RT-PCR Assays for Selective Detection of Human Rhinovirus

This invention relates to selective detection of human rhinovirus (HRV) in biological media. Specifically, this invention discloses a real-time TaqMan RT-PCR assay targeting the 5'-noncoding region of the HRV genome. This is a one-step, real-time nucleic acid assay that offers rapid, sensitive, and quantitative results. The assay is validated against all 100 recognized HRV prototype strains.

HRV is the most frequent cause of the common cold. From a clinical standpoint, diagnosis of HRV infection is quite difficult as the related symptoms can be caused by other agents as well. Additionally, laboratory detection of HRV is challenging as HRV exhibits extreme antigenic variability and certain strains cannot be maintained by cell culture.

Potential Commercial Applications:		Competitive Advantages:
Development of human rhinovirus (HRV) diagnostics Acute lower respiratory illness diagnostics and investigation		Validated against all 100 human rhinovirus prototype strains Rapid, sensitive and quantitative One-step assay Easily adapted to kit form

Development Stage:

Early-stage

In vitro data available

Inventors:

Xiaoyan Lu (CDC)  ➽ more inventions...

Dean Erdman (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 8,986,933 issued 2015-03-24
U.S. Pat: 9,650,685 issued 2017-05-16

Publications:
Lu X, et al. PMID 18057136

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-177-2013-0
Updated: Aug 13, 2013