A method to label heparan sulfate proteoglycan in the plasma membrane of mammalian cells
Heparan sulfate proteoglycan (HSPG) is a group of lipid-anchored proteoglycans, engaged in a variety of key biological functions on cell surface. HSPG-mediated endocytosis of neurotoxic protein aggregates has been linked to aging related neurodegenerative diseases. Labeling HSPG is a promising technique to trace cell profile in cell research, monitor its trafficking in live cells and in tissues. Researchers at the NIDDK have discovered a method in which a positively charged fluorescent protein binds specifically to HSPG on cell surface. This is a promising cost-effective technique that will help scientists to monitor the fate of HSPG in real time on live cell surface as well as to rapidly label the cell boundary-frequently required in cell biology research.
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Inventors:
Qi Zhang (NIDDK) ➽ more inventions...
Yihong Ye (NIDDK) ➽ more inventions...
Intellectual Property:
Publications:
Zhang Q, et al A myosin-7B–dependent endocytosis pathway mediates cellular entry of α-synuclein fibrils and polycation-bearing cargos. PNAS May 19, 2020 117 (20) 10865-10875 doi 10.1073/pnas.1918617117
Collaboration Opportunity:
The NIDDK is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize Method to Label HSPG in Mammalian Cells. For collaboration opportunities, please contact: Dr. Yihong Ye at yihongy@mail.nih.gov
Licensing Contact:
Betty Tong, Ph.D.
Email: tongb@niddk.nih.gov
Phone: 301-451-7836
OTT Reference No: E-143-2019-0
Updated: Aug 13, 2020