A method to label heparan sulfate proteoglycan in the plasma membrane of mammalian cells


Heparan sulfate proteoglycan (HSPG) is a group of lipid-anchored proteoglycans, engaged in a variety of key biological functions on cell surface. HSPG-mediated endocytosis of neurotoxic protein aggregates has been linked to aging related neurodegenerative diseases. Labeling HSPG is a promising technique to trace cell profile in cell research, monitor its trafficking in live cells and in tissues. Researchers at the NIDDK have discovered a method in which a positively charged fluorescent protein binds specifically to HSPG on cell surface. This is a promising cost-effective technique that will help scientists to monitor the fate of HSPG in real time on live cell surface as well as to rapidly label the cell boundary-frequently required in cell biology research.

Potential Commercial Applications: Competitive Advantages:
  • This technology will be useful to the researchers working on HSPG function in mammalian cells
  • This technology will benefit cell biologists in general to quickly label the cell boundary while doing cell imaging
  • This technology will be useful to researchers working in the field of aging related neurodegenerative studies
 
  • This technology allows a rapid way to detect HSPG on cell surface in live cells
  • This is a faster and more cost effective technology which can also be used in live cell imaging to track HSPG-dependent endocytosis of cargo proteins compared to the current practices which use HSPG antibodies to label HSPG
  • This technology offers a quick method to label the cell boundary-often required in cell biology research


Inventors:

Qi Zhang (NIDDK)  ➽ more inventions...

Yihong Ye (NIDDK)  ➽ more inventions...


Intellectual Property:

Publications:
Zhang Q, et al A myosin-7B–dependent endocytosis pathway mediates cellular entry of α-synuclein fibrils and polycation-bearing cargos. PNAS May 19, 2020 117 (20) 10865-10875 doi 10.1073/pnas.1918617117

Collaboration Opportunity:

The NIDDK is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize Method to Label HSPG in Mammalian Cells. For collaboration opportunities, please contact: Dr. Yihong Ye at yihongy@mail.nih.gov


Licensing Contact:
Betty Tong, Ph.D.
Email: tongb@niddk.nih.gov
Phone: 301-451-7836

OTT Reference No: E-143-2019-0
Updated: Aug 13, 2020