Novel Fixative for Improved Biomolecule Quality from Paraffin-Embedded Tissue

Tissues samples collected during medical procedures, such as biopsies, are used to diagnose a wide variety of diseases. Before diagnosis, patient samples are typically processed by fixation and paraffin embedding. This fixation/embedding process is used to preserve tissue morphology and histology for subsequent evaluation. Unfortunately, most fixative agents can damage or destroy nucleic acids (RNA and DNA) and damage proteins during the fixation process, thereby potentially impairing diagnostic assessment of tissue.

Researchers in the National Cancer Institute’s Laboratory of Pathology have developed an improved tissue fixative solution that is formaldehyde-free. This novel fixative, BE70, significantly improves DNA, RNA, and protein biomolecule integrity in histological samples compared to traditional fixatives. Additionally, BE70 is compatible with current protocols and does not alter tissue processing.

Potential Commercial Applications: Competitive Advantages:
  • Improve integrity of fixed tissue samples
  • Improve RNA/DNA quality in fixed tissue samples
  • Non-cross linking, improved protein quality
  • There is substantial interest in new fixatives to replace neutral buffered formalin (a carcinogen) as primary fixative agent for surgical pathology
  • BE70 overcomes several limitations of other fixatives, including cost and disposal issues
  • Could be formulated as a concentrate, and marketed as an additive (to be added during dilution of ethanol)

Development Stage:
Pre-clinical (in vivo)


Stephen Hewitt (NCI)  ➽ more inventions...

Joon-Yong Chung (NCI)  ➽ more inventions...

Candice Perry

Intellectual Property:
US Provisional Application No. 62/255,030

Perry C, et al. A Buffered Alcohol-Based Fixative for Histomorphologic and Molecular Applications. PMID 27221702

Collaboration Opportunity:

Licensing only

Licensing Contact:
John Hewes, Ph.D.
Phone: 240-276-5515

OTT Reference No: E-139-2015
Updated: Jul 19, 2018