Tg(MMTV-Cre)#Mam Mouse Model for Developmental Biology, Hepatology, and Oncology Studies


Cre-recombinase under the control of mouse mammary tumor virus long terminal repeat (MMTV) was expressed in the salivary gland and mammary epithelial cells of adult mice, and induced recombination in all tissues.

The Cre recombinase from bacteriophage P1 excises intervening DNA sequences located between two unidirectional lox sites positioned on the same linear DNA segment, leaving one lox site behind. Through insertion of lox sites via homologous recombination into the gene of interest and targeting Cre recombinase expression to a specific cell type using a tissue-specific promoter, it is possible to introduce predetermined deletions into the mammalian genome. To delete genes specifically from mammary gland, transgenic mice were created carrying the Cre gene under the control of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR). In adult MMTV-Cre mice, expression of the transgene was confined to striated ductal cells of the salivary gland and mammary epithelial cells in virgin and lactating mice. In contrast to WAP-Cre, however, Cre expression under control of the MMTV LR resulted in recombination in all tissues.

Potential Commercial Applications: Competitive Advantages:
Developmental Biology
Hepatology
Oncology
 


Development Stage:
Pre-clinical

Inventors:

Lothar Hennighausen (NIDDK)  ➽ more inventions...


Intellectual Property:
Research Tool — Patent protection is not being pursued for this technology.

Publications:
Wagner KU, et al. PMID 9336464

Licensing Contact:
Betty Tong, Ph.D.
Email: tongb@niddk.nih.gov
Phone: 301-451-7836

OTT Reference No: E-113-2012-0
Updated: Apr 17, 2017