Rabbit Polyclonal Antiserum to the Nitrone Spin Trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO)


Biological radicals resulted from oxidative stress has been implicated in human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of either radical formation, the end-products of radical formation or susceptibility for radical formation. The Rabbit polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy. This rabbit polyclonal anti-DMPO antiserum will replace a previously licensed one which is no longer available (E-186-2003). The availability of anti-DMPO from three different sources will greatly expand the ability of researchers to use anti-DMPO in conjunction with other antibodies.

Potential Commercial Applications: Competitive Advantages:
  • The Rabbit polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy.
 
  • DMPO is one of the most frequently used spin traps to detect free radicals and has been cited in over 1000 articles (PubMed). The availability of anti-DMPO of different origins (rabbit, mouse) will increase the ability of the researcher to use anti-DMPO in conjunction with other antibodies of interest.


Inventors:

Ronald Mason (NIEHS)  ➽ more inventions...

Marilyn Ehrenshaft (NIEHS)  ➽ more inventions...


Intellectual Property:
Research Tool – Patent protection is not being pursued for this technology.

Licensing Contact:
Vidita Choudhry, Ph.D.
Email: vidita.choudhry@nih.gov
Phone: 301-594-4095

OTT Reference No: E-109-2013-0
Updated: Aug 6, 2015