Chicken Polyclonal Antiserum to the Nitrone Spin Trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO)

Biological radicals resulted from oxidative stress has been implicated in human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of either radical formation, the end-products of radical formation or susceptibility for radical formation. The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy. This chicken polyclonal anti-DMPO antiserum will supplement both the mouse monoclonal anti-DMPO and the rabbit polyclonal anti-DMPO. The availability of anti-DMPO from three different sources will greatly expand the ability of researchers to use anti-DMPO in conjunction with other antibodies.

Potential Commercial Applications:		Competitive Advantages:
The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy.		DMPO is one of the most frequently used spin traps to detect free radicals and has been cited in over 1000 articles (PubMed). The availability of anti-DMPO of different origins (rabbit, mouse, chicken) will increase the ability of the researcher to use anti-DMPO in conjuction with other antibodies of interest.

Inventors:

Ronald Mason (NIEHS)  ➽ more inventions...

Marilyn Ehrenshaft (NIEHS)  ➽ more inventions...


Intellectual Property:
Research Tool – Patent protection is not being pursued for this technology.

Licensing Contact:
Vidita Choudhry, Ph.D.
Email: vidita.choudhry@nih.gov
Phone: 301-594-4095

OTT Reference No: E-108-2013-0
Updated: Aug 6, 2015