Recombinant Stabilized Prefusion Protein of Respiratory Syncytial Virus for Use as a Subunit Vaccine
The invention, a stabilized recombinant prefusion F protein (pre F), is a candidate subunit vaccine for Respiratory Syncytial Virus (RSV). Pre-F is stabilized in the prefusion conformation and displays epitopes not present in postfusion F protein. Several potent RSV neutralizing antibodies bind pre F, but not postfusion F. Therefore, immunization with pre F may elicit an immune response superior to the response generated by postfusion F.
NIH researchers have engineered pre F to expose an antigenic site 0, which is targeted by extremely potent RSV neutralizing antibodies. Structure-based design yielded several stabilized variants of pre F that maintained exposure of antigenic site 0 when subjected to extremes of pH, osmolality and temperature.
Preclinical in vivo data on stabilized pre F is available. Immunization of mice and macaques with antigenic site 0 stabilized pre F variants elicited high levels of RSV specific neutralizing activity.
Potential Commercial Applications: | Competitive Advantages: |
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Development Stage:
- Pre-clinical In vivo data available (animal)
Inventors:
Michael Joyce (NIAID) ➽ more inventions...
Jason McLellan (NIAID) ➽ more inventions...
Barney Graham (NIAID) ➽ more inventions...
Peter Kwong (NIAID) ➽ more inventions...
Baoshan Zhang (NIAID) ➽ more inventions...
Masaru Kanekiyo (NIAID) ➽ more inventions...
Man Chen (NIAID) ➽ more inventions...
Jeffrey Boyington (NIAID) ➽ more inventions...
Gwo-Yu Chuang (NIAID) ➽ more inventions...
Ivelin Georgiev (NIAID) ➽ more inventions...
Jason Gorman (NIAID) ➽ more inventions...
Gilad Ofek (NIAID) ➽ more inventions...
Marie Pancera (NIAID) ➽ more inventions...
Mallika Sastry (NIAID) ➽ more inventions...
Yongping Yang (NIAID) ➽ more inventions...
Sanjay Srivatsan (NIAID) ➽ more inventions...
Tongqing Zhou (NIAID) ➽ more inventions...
Lei Chen (NIAID) ➽ more inventions...
Cinque Soto (NIAID) ➽ more inventions...
Guillaume Stewart-Jones (NIAID) ➽ more inventions...
Intellectual Property:
U.S. Pat: 9,738,689 issued 2017-08-22
U.S. Pat: 10,017,543 issued 2018-07-10
US Application No. 61/780,910
US Application No. 61/798,389
US Application No. 61/857,613
US Application No. 61/863,909
PCT Application No. PCT/US2014/026714
US Application No. 15/633,578
US Application No. 16/025,858
Publications:
McLellan JS, et al. PMID 23618766
McLellan JS, et al. PMID 24179220
Licensing Contact:
Carol Salata, Ph.D.
Email: csalata@mail.nih.gov
Phone: 240-627-3727
OTT Reference No: E-081-2013-0
Updated: Jun 22, 2018