Method to Remove Mycoplasma Contamination from Virus Stocks


Mycoplasma are a form of bacteria that are commonly found as contaminants in cell cultures. They adversely affect cell line growth rates and viral vaccine production. Mycoplasma contamination is a challenge for the vaccine industry and virology researchers. Current commercial reagents or kits only temporarily inhibit the growth of mycoplasma, but cannot eliminate the contaminants.

CDC has developed a method that can reliably eliminate mycoplasma from enveloped virus stock. This method allows for the removal of the mycoplasma without harming harvested virus. The harvested virus can then be transfected back into a clean cell line for continued virus production. Although designed for purification of rabies virus stocks, the method may successfully remove mycoplasma contamination from stocks of other enveloped viruses such as measles, mumps, respiratory syncytial virus, and influenza virus. Additional in vitro testing is planned for other virus purification. Rabies strains treated using this method are available.

Potential Commercial Applications: Competitive Advantages:
  • Restore a clean vaccine repository from historical old strains for vaccine research and development
  • A kit to selectively remove mycoplasma from virus seed stocks
  • Research tool for virus study
 
  • Permanent removal of mycoplasma
  • Increased cell growth rate and improved vaccine production


Related Invention(s):
E-040-2018-0
E-099-2018-0


Inventors:

Xianfu Wu (CDC)  ➽ more inventions...

Victoria Olson (CDC)  ➽ more inventions...

Yong Yang (CDC)  ➽ more inventions...


Intellectual Property:
US Application No. 62/645,002

Collaboration Opportunity:

The CDC Technology Transfer Office (TTO) is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize: Method to Remove Mycoplasma Contamination from Virus Stocks. For collaboration opportunities, please contact CDC TTO at tto@cdc.govor 1-404-639-1330.


Licensing Contact:
Jeremiah Mitzelfelt,
Email: jeremiah.mitzelfelt@nih.gov
Phone: 301-443-8518

OTT Reference No: E-039-2018-0
Updated: Jun 13, 2018