C57BL/6J Embryonic Stem Cell Lines Generated Using Serum-Free Media

NIH investigators have generated Embryonic Stem (ES) cell clones from C57BL/6J mice in a defined medium. These cell lines enable direct genetic alteration of mice in a pure genetic background.

Using a defined media supplement, knockout serum replacement (KSR) with knockout DMEM (KSR-KDMEM), the investigators established ES cell lines from blastocysts of C57BL/6J mice. One specific cell line, HGTC-8 was found to be karyotypically stable and germline competent, both prior to manipulation and after gene targeting. These cell lines transfected more efficiently, and exhibited increased efficiencies of cell cloning and chimera generation, when maintained in KSR-KDMEM.

Potential Commercial Applications: Competitive Advantages:
  • Generation of knockout mice without the need to backcross.
  • Generation of mice via targeted mutations.


Jun Cheng (NHGRI)  ➽ more inventions...

Pamela Schwartzberg (NHGRI)  ➽ more inventions...

Intellectual Property:
Research Tool -- patent protection is not being pursued for this technology


J Cheng, A Dutra, A Takesono, L Garrett-Beal, PL Schwartzberg. Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media. Genesis. 2004 June; 39(2):100-104.

PubMed abs

Licensing Contact:
Eggerton Campbell, Ph.D.
Email: eggerton.campbell@nih.gov
Phone: 301-402-1648

OTT Reference No: E-038-2009-0
Updated: May 15, 2009