Cells Expressing Both Human CD4 And A Human Fusion Accessory Factor Associated With HIV Infection


HIV-1 infects target cells by first binding to CD4, a receptor on the target cell membrane. The virus and target cell membranes then fuse, allowing the virus to enter the target cell. It has previously been determined that CD4 alone is not sufficient to allow entry, but that another factor specific to human cells is also required. The current invention embodies the identification of a cDNA encoding a protein, designated "fusin," which demonstrates properties expected of a fusion co-factor for T-cell line tropic HIV-1 isolates. Fusin is a member of the 7-transmembrane segment (7-TMS) superfamily of G-protein-coupled receptors. While this cDNA has previously been cloned, its potential role as an accessory protein necessary for HIV infection is novel to the current invention. The invention, therefore, should represent a valuable tool to be used in the production of transgenic mice and of cell lines for the study of HIV infection. In addition, the invention may itself represent a potential therapeutic agent against HIV or target for agents acting to block entry of HIV into target cells. This technology was reported in Science 272:809-810 (1996); Chemical and Engineering News, p. 7 (May 13, 1996); BioWorld Today, pp. 1-2 (May 13, 1996); Biotechnology News, 16(13):1-2 (1996); and BioWorld Today, pp. 1,3 (June 21, 1996).

Inventors:

Edward Berger (NIAID)  ➽ more inventions...

Christopher Broder (NIAID)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 6,197,578 issued 2001-03-06
US Application No. PCT/US97/00956
PCT Application No. 09/722,779

Publications:
O Nussbaum et al., J. Virol., 68(9):5411-5422 (1994)
C Broder et al., PNAS 92:9004-9008 (1995)
Y Fent et al., Science 272:872-877 (1996)

Licensing Contact:
Benjamin Hurley, Ph.D.
Email: benjamin.hurley@nih.gov
Phone: 240-669-5092

OTT Reference No: E-017-1996-1
Updated: Nov 1, 1996