Fluorescent Intracellular Calcium Indicators

Calcium is a key element in the regulation of many cellular processes, including muscle contraction, hormone excretion from gland cells, neurotransmitter release from nerve synapses, and the regulation of cellular metabolism. Elevated calcium levels are found in a number of diseases.

The present invention relates to chromophoric or fluorescent dye calcium indicators that are superior for measurement of high concentrations of calcium ions due to their high dissociation constants. As a result of the high calcium ion dissociation constants, the perturbation resulting from introducing the indicator into the cell is greatly reduced. These calcium ion indicators can be measured by various techniques including 19F NMR spectroscopy, flow cytometry, and quantitative fluorescence techniques, and are useful for measuring calcium levels within the cytosol or within cellular organelles.

Potential Commercial Applications: Competitive Advantages:
Research tool for quantifying intracellular calcium concentrations. 


Robert London (NIEHS)  ➽ more inventions...

Louis Levy (NIEHS)  ➽ more inventions...

Intellectual Property:
US Pat: 5,516,911 issued 1996-05-14

Collaboration Opportunity:

The NIEHS Laboratory of Structural Biology is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize this technology. Please contact Dr. Robert London at 919/541-4879 or london@niehs.nih.gov for more information.

Licensing Contact:
Vidita Choudhry, Ph.D.
Email: vidita.choudhry@nih.gov
Phone: 301-594-4095

OTT Reference No: E-015-1993-0
Updated: Mar 1, 2007