Technology ID
E-041-2004-0

Codon-optimization of HIV-1 Viral Infectivity Factor (VIF) Gene

Linked ID
TAB-846
Inventors
Kim-Lien Nguyen (NIAID)
Klaus Strebel (NIAID)
Stephan Bour (NIAID)
Lead Inventors
Klaus Strebel (NIAID)
Co-Inventors
Kim-Lien Nguyen (NIAID)
Stephan Bour (NIAID)
Applications
Research Materials
Therapeutic Areas
Infectious Disease
ICs
NIAID
Commercial Applications
  • A plasmid pcDNA-hVif capable of expressing VIF independent of other HIV genes through removal of inhibitory sequences on its mRNA.
Expression of the HIV-1 Vif protein in the absence of other viral factors such a Tat and Rev is extremely inefficient due to the presence of inhibitory sequences on its mRNA. This invention uses codon optimization to remove such inhibitory sequences without altering the amino acid sequence of the protein. The modified vif gene in the resulting pcDNA -hVIF vector is expressed under the control of the CMV promoter. In this, the protein functions as wild type and is more amendable to high-level expression in mammalian cells.

Currently this vector is used in on-going studies of HIV infection and its ability to overcome cellular restriction to replication. As such, the reagent will be valuable to other researchers in discovering mechanisms of replication, next generation therapeutics and potentially prevention of infection as well.

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