Researchers at the National Cancer Institute developed silicone hydrogel support structures that mimic tissue vasculature (e.g., capillary bed) with high oxygen diffusivity. Photolithographic methods are used to construct mimetic silicone hydrogel pillars that have, for example, a 20:1 height to diameter ratio. Advantageously, these mimetic silicone hydrogels diffuse oxygen from the bottom chamber to the cells cultured on the surface at near physiological rates (60 times that of water).
A variety of techniques have been used to isolate specific cells or cell populations from a histological sample under direct microscopic visualization. Techniques that involve manual or micro-manipulation devices to isolate individual cells based upon visible characteristics and/or immunological staining are labor intensive and can require an extended amount of time.
Researchers at the NCI Radiation Oncology Branch and NIH CIT Center for Molecular Modeling developed a tetrahydroxamate chelation technology that provides a more-stable Zr-89 complex as an immuno-PET cancer imaging agent. In either the linear or the macrocyclic form, the tetrahydroxamate complexes exhibit greater stability as chelating agents compared to Zr-89 complexed to the siderophore desferrioxamine B (DFB), a trihydroxamate, which represents the current s