A Transgenic Mouse Model for Tetracycline Regulation of Active TGFbeta1 in Mice: tetO TGFbeta1

Many human cancers and other skin ailments arise from overexpression of the polypeptide TGFbeta1 growth factor. This growth factor is a growth inhibitor whose function involves cell differentiation and development. It is thought that overexpression of this protein is a contributing factor in many diseases, including certain cancers and dermal fibrosis.

There is a need for mouse models that can exhibit overexpression of TGFbeta1 in a locally specific manner. Such is the technology being made available. The technology relates to a mouse model where the overexpression in epithelial cells is achieved via the bigenic tetracycline regulatory system. Expression of tetO TGFbeta1 occurs when the mice are bred with a second transgenic line expressing the transactivator tTa or rTa. The rTa or tTa have been coupled with keratin 5 promoters, enabling localized activation of tetO TGFbeta1 in the presence or absence of tetracyclines upon successful mating. The potential uses of these models is invaluable and can assist similar research involving different tissue specificity.


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Updated: Jun 16, 2010