Technology Bundle ID: TAB-3231

Monoclonal Antibody Specific for DNA/RNA Hybrid Molecules

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Licensing Contact:

Wade W Green
wade.green@nih.gov
Phone: 301-761-7505

Primary Inventors: 
Stephen Leppla (NIAID)
Co-Inventors: 
Clinton Leysath (NIAID), Damilola Phillips (NIAID), David Garboczi (NIAID), Larry Lantz (NIAID)
Therapeutic Area: 
Infectious Disease
Application: 
Research Materials
Institute or Center: 
NIAID

NIAID has a hybridoma available for non-exclusive licensing that produces a monoclonal antibody specific for DNA/RNA hybrids. This antibody, which has been extensively characterized by NIH researchers, is already a widely-used research tool. It is currently the only monoclonal antibody available that is specific for DNA/RNA hybrids, making it a unique reagent. It is used in immuno-fluorescence (IF) microscopy, where it can be used to detect sites of transcriptional activity and potentially sites of viral replication. It has also been used in DNA/RNA immunoprecipitation (DRIP) experiments by a variety of researchers.

Aside from its use as a research tool, this antibody has potential to be used in diagnostic kits for viral/bacterial infections, cancers, and a variety of other human diseases. DNA/RNA hybrids arise during normal cellular function, but they are typically present in cells at low levels. When DNA/RNA hybrids are found at high levels in a cell, it indicates that the cell is "abnormal". For example, the cell may be cancerous or infected with a virus. NIH researchers have also incorporated the antibody into a micro-array platform, expanding its potential for use in diagnostic devices.

This technology is available for licensing for commercial development in accordance with 35 U.S.C. 209 and 37 CFR Part 404, as well as for further development and evaluation under a research collaboration.

Applications:

Research tool:

  • Detection and visualization of DNA/RNA hybrids, "R-loops", or sites of viral replication in cells
  • DNA/RNA immunoprecipitation (DRIP) studies
  • Antibody based micro-arrays

For use in diagnostic kits that detect:

  • Viral/bacterial infections
  • miRNA biomarkers of disease (i.e. certain cancers)
Advantages:
  • Only available monoclonal antibody specific for DNA/RNA hybrids
  • Binding properties extensively characterized by NIH researchers
  • Widely-accepted as a key research reagent
  • Antibody based micro-arrays are inexpensive, efficient, and increase detection of small or structured transcripts, as well as transcripts present at low levels

Patents

EIR Application

Research Tool – Patent protection is not being pursued for this technology.

Publications

Hu Z, et al.
PMID 16614443
Phillips DD, et al.
PMID 23784994

Updated

Dec 15, 2017

Data Source: 
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