Technology Bundle ID: TAB-2648

Real-time PCR Multiplex Assay for Detection of Bacterial Respiratory Pathogens in Clinical Specimens

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Licensing Contact:
Primary Inventors: 
Jonas Winchell (CDC)
Co-Inventors: 
Agnes Warner (CDC), Kathleen Thurman (CDC)
Therapeutic Area: 
Infectious Disease
Application: 
Diagnostics
Development Status: 
  • Pre-clinical
  • In vitro data available
Institute or Center: 
CDC

CDC researchers have developed a single-tube, real-time PCR assay for the simultaneous detection of three bacterial respiratory pathogens (Mycoplasma pneumoniae, Chlamydiophila pneumoniae and Legionella spp.). The assay has an internal control testing for presence of human DNA. This four-plex real-time PCR assay could potentially become a routine screening test for patients with respiratory illness. Ninety four clinical specimens (in a 96-well format) can be tested at once. This assay is non-invasive, rapid and cost-effective. It has the potential for point-of-care applications in population-based pneumonia surveillance.

Applications:
  • Population-based pneumonia surveillance
  • Development of broadly-capable respiratory clinical diagnostics
Advantages:
  • Sensitive and specific
  • High-throughput friendly
  • Rapid and cost-effective compared to screening for individual respiratory pathogens
  • Easily developed for use in diagnostic kits

Patents

PCT Application PCT/US2011/032749
Filed on 2011-04-15
US Application 61/324,986
Filed on 2010-04-16
US Pat 9,260,762

Issued 2016-02-16

Publications

Thurman KA, et al.
PMID 21397428

Updated

Sep 19, 2013

Data Source: 
tts