Technology Bundle ID: TAB-2637

sodC-Based Real-Time PCR Assay for Detection of Neisseria meningitidis Infection

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Licensing Contact:
Primary Inventors: 
Jennifer Thomas (CDC)
Therapeutic Area: 
Infectious Disease
Application: 
Diagnostics
Development Status: 
  • Early-stage
  • In vitro data available
Institute or Center: 
CDC

CDC researchers have developed a real-time PCR assay for the detection of Neisseria meningitidis sodC within clinical specimens. The ability to detect all strains of N. meningitidis, regardless of individual serogroup, is the central innovation of this technology. Further, the assay is sensitive enough to detect even the very limited sample sizes of N. meningitidis that would typically be found in clinical specimens. This technology avoids potentially catastrophic false-negative results associated with current N. meningitidis carriage study testing methods. At least 16% of carried N. meningitidis lacks the ctrA gene, which is the current target of serogroup-based real-time PCR. N. meningitidis is the etiologic agent of epidemic bacterial meningitis and sepsis throughout the world and rapid detection of N. meningitidis infection is essential for patient well-being.

Applications:
  • Routine N. meningitis surveillance, especially useful in carriage studies
  • Rapid, specific identification of N. meningitis infection
Advantages:
  • Rapid, sensitive and specific
  • Present culture detection methods are limited by low sensitivity and long incubation periods; this assay demonstrates improved detection of meningococci, regardless of encapsulation status or bacteria viability
  • Circumvents ctrA-based testing-related false negative results in carriage studies
  • No further technical development needed for commercialization

Patents

PCT Application PCT/US2011/055784
Filed on 2011-10-11
US Application 15/251,507
Filed on 2016-08-30
US Application 61/391,493
Filed on 2010-10-11
US Pat 9,487,832

Issued 2016-11-08

Publications

Dolan TJ, et al.
PMID 21573213

Updated

Sep 17, 2013

Data Source: 
tts