Technology Bundle ID: TAB-2612

Real-time TaqMan RT-PCR Assays for Selective Detection of Human Rhinovirus

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Licensing Contact:
Primary Inventors: 
Xiaoyan Lu (CDC)
Co-Inventors: 
Dean Erdman (CDC)
Therapeutic Area: 
Infectious Disease
Application: 
Diagnostics
Development Status: 
  • Early-stage
  • In vitro data available
  • Institute or Center: 
    CDC

    This invention relates to selective detection of human rhinovirus (HRV) in biological media. Specifically, this invention discloses a real-time TaqMan RT-PCR assay targeting the 5'-noncoding region of the HRV genome. This is a one-step, real-time nucleic acid assay that offers rapid, sensitive, and quantitative results. The assay is validated against all 100 recognized HRV prototype strains.

    HRV is the most frequent cause of the common cold. From a clinical standpoint, diagnosis of HRV infection is quite difficult as the related symptoms can be caused by other agents as well. Additionally, laboratory detection of HRV is challenging as HRV exhibits extreme antigenic variability and certain strains cannot be maintained by cell culture.

    Applications:
    • Development of human rhinovirus (HRV) diagnostics
    • Acute lower respiratory illness diagnostics and investigation
    Advantages:
    • Validated against all 100 human rhinovirus prototype strains
    • Rapid, sensitive and quantitative
    • One-step assay
    • Easily adapted to kit form

    Patents

    US Pat 8,986,933

    Issued 2015-03-24
    US Pat 9,650,685

    Issued 2017-05-16

    Publications

    Lu X, et al.
    PMID 18057136

    Updated

    Aug 13, 2013

    Data Source: 
    tts