Technology Bundle ID: TAB-2600

Real-Time RT-PCR Assay for Detection of Noroviruses

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Licensing Contact:
Primary Inventors: 
Jan Vinje (CDC)
Co-Inventors: 
Leslie Barclay (CDC), Nicole Gregoricus (CDC), Preeti Chhabra (CDC)
Therapeutic Area: 
Infectious Disease
Application: 
Diagnostics
Development Status: 
  • Pre-clinical
  • In vitro data available
Institute or Center: 
CDC

A specific and sensitive TaqMan-based real-time (rt) RT-PCR assay has been developed by CDC scientists for detection of noroviruses in clinical and environmental specimens. This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. Additionally, the assay is multiplexed with an internal extraction control virus (coliphage MS2) to validate the results of the assay. Since the virus cannot be grown in cell culture and enzyme immunoassays lack the necessary sensitivity, this technology is particularly useful.

Applications:
  • Development of norovirus diagnostics
  • Specific rtRT-PCR assay for detecting and distinguishing of the major pathogenic norovirus genogroups (I and II) within clinical and environmental samples
Advantages:
  • This is an internally controlled, multiplexed assay capable of rapid, accurate identification of norovirus genogroups responsible for human illness
  • Superior sensitivity compared with immunoassay detection methods

Patents

PCT Application PCT/US2012/065269
Filed on 2012-11-15
US Application 14/358,493
Filed on 2014-05-15
US Application 61/560,077
Filed on 2011-11-15

Publications

Schultz AC, et al.
PMID 21195660
Vega E, et al.
PMID 21801614

Updated

Aug 2, 2013

Data Source: 
tts