Technology Bundle ID: TAB-2471

Plasmid Useful in Transplantation Therapy for Age-Related Eye Disease

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Primary Inventors: 
Kapil Bharti (NINDS)
Application: 
Research Materials
Development Status: 
  • Prototype
  • In vitro data available
Institute or Center: 
NINDS

Researchers have developed a green fluorescent protein (GFP) based plasmid that can be used to detect differentiated retinal pigment epithelium (RPE) cells. RPE is a layer of cells located behind the eye that becomes damaged in age-related macular degeneration (AMD). Current cell based therapies for treating AMD focus on generating RPE cells from stem cells. This GPF-based plasmid can be inserted into growing stem cells, and the fluorescence marker can be used to detect and purify stem cells differentiating into RPE cells. This advancement allows generation of a purified population of RPE cells for in vitro and transplantation purposes.

Additionally, cells comprising the GFP-based construct may be useful in high-throughput drug screening as a means to: (1) identify potential therapeutic targets of RPE degenerative diseases such as AMD, and (2) evaluate initial toxicity of candidate drugs in RPE cells.

Applications:
  • Fluorescence based marker for detecting and purifying differentiated RPE cells
  • Potential use in high throughput drug screening
Advantages:

GFP based marker allows for fast and simple detection of differentiated RPE cells from stem cells.

Patents

EIR Application

Research Tool — Patent protection is not being pursued for this technology.

Publications

Bharti K, et al.
PMID 20846177

Updated

Aug 10, 2012

Data Source: 
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