Technology Bundle ID: TAB-1589

A Shuttle Plasmid, Recombinant MVA/HIV1 Clinical Vaccine Constructs and a Mechanism for Enhanced Stability of Foreign Gene Inserts by Codon Alternation and for Insertion of the Foreign Gene Between Two Vaccinia Virus Essential Genes

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Licensing Contact:
Primary Inventors: 
Bernard Moss (NIAID)
Co-Inventors: 
Linda Wyatt (NIAID), Patricia Earl (NIAID)
Therapeutic Area: 
Infectious Disease
Application: 
Research Materials
Development Status: 

Vaccine candidates have been synthesized and preclinical studies have been performed. The vaccine candidates of this invention are slated to enter Phase I clinical trials in the next year.

Institute or Center: 
NIAID

Since the onset of the AIDS epidemic more than two decades ago, enormous efforts have been directed to making a vaccine that will protect against human immunodeficiency virus-1 (HIV); an effective vaccine is thought to require the induction of cellular and humoral responses. Vaccine candidates have included a variety of HIV immunogens delivered as DNA, attenuated poxviruses, adenoviruses, vesicular stomatitis virus, proteins, and various combinations thereof. The inventors' efforts to design an HIV vaccine have focused on modified vaccinia virus Ankara (MVA) as a vector.

The patent application describes (1) the shuttle plasmid, pLW73, used for insertion of a foreign gene between two essential vaccinia virus genes (in this case, I8R, G1L), (2) an MVA/Ugandan Clade D (UGD) construct, and (3) an MVA/HIV 75 AG construct using pLW73 as a vector. Additionally, the invention provides two methods: (1) a method useful for large-scale production of recombinant vaccinia viruses, and (2) a method for stabilizing foreign gene inserts that undergo mutation after repeated passages, again useful in large-scale production of recombinant vaccinia viruses.

Applications:
  • Immunization against HIV

Patents

PCT Application PCT/IB2007/004575
Filed on 2007-08-24
US Application 16/579,276
Filed on 2019-09-23
US Application 60/840,093
Filed on 2006-08-25
US Pat 10,421,978

Issued 2019-09-24
US Pat 9,133,478

Issued 2015-09-15

Updated

Aug 1, 2007

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